Primer Design and Search Tool

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Primer search, ePCR

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Statistics

Visitors:

1196949

Primer tm:

96133

Primer score:

22313/14401

Search:

17927/13143

Fast PCR:

3276730/1313924

Primer design:

106129/121937

MSP design:

35336

Version:

2.75

Last update:

21/03/23

Search for the best primer pairs

Sequence
Bisulfite
Set search region for	Forward primer: - Reverse primer: -
Max length of PCR

Parameters

Primer melting temperature

Primer conc	mikromol	Glycerol conc	%
Potassium conc	milimol	Ethylen glycol conc	%
Magnesium conc	milimol	Formamid conc	%

Primer scoring values

Description	Weight	Min	Opt	Max		Description	Weight	Max
Primer length						Self annealing
GC content						Self end-annealing
GC content (bis)						Pair annealing
Melting temp						Pair end-annealing

Primer design

Results list size

Max Tm diff

Minimum of CpGs

Database search and fast PCR

Database		Mismatches	Max:
PCR length		PCR product to show
		Primer matches to show

Help

Search for the best primer pairs

Online help system

The dynamic Online help system informs you about each input field. Move the mouse over the input field you would like to fill in, and read the help here.

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