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Enzyme Information: T4 DNA Ligase
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| Product name |
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T4 DNA Ligase |
| Cat# |
: |
EL0015 |
| Unit size |
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200u |
| EC# |
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6.5.1.1 |
| CAS# |
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9015-85-4 |
| Concentration |
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1u/µl |
| Mol.weight |
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55.3kDa monomer. |
| Applications |
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Joining double-stranded DNA with cohesive or blunt termini
Joining of oligonucleotide linkers or adaptors to blunt-ended DNA
Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids
Ligase-mediated RNA detection
Site-directed mutagenesis |
| Source |
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E.coli cells with a cloned gene 30 of bacteriophage T4. |
| Storage |
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The enzyme is supplied in: 20mM Tris-HCl (pH 7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA and 50% (v/v) glycerol. |
| Description |
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The T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesive-end termini. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids but has no activity on single-stranded nucleic acids.
The T4 DNA Ligase requires ATP as cofactor |
| Request Info |
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| Supplier |
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Fermentas Inc. |
| Contact info |
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7520 Connelley Drive
Hanover. MD , United States
Phone:800 340 9026 |
Scientific Information: EC 6.5.1.1
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| EC No |
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6.5.1.1 |
| Accepted Name |
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DNA ligase (ATP) |
| Reactions |
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ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + diphosphate + (deoxyribonucleotide)n+m |
| Other Names |
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polydeoxyribonucleotide synthase (ATP); polynucleotide ligase; sealase; DNA repair enzyme; DNA joinase; DNA ligase; deoxyribonucleic ligase; deoxyribonucleate ligase; DNA-joining enzyme; deoxyribonucleic-joining enzyme; deoxyribonucleic acid-joining enzyme; deoxyribonucleic repair enzyme; deoxyribonucleic joinase; deoxyribonucleic acid ligase; deoxyribonucleic acid joinase; deoxyribonucleic acid repair enzyme |
| Systematic Name |
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oly(deoxyribonucleotide):poly(deoxyribonucleotide) ligase (AMP-forming) |
| CAS# |
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9015-85-4 |
| External Links |
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NCBI:
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| PubMed, Protein, Nucleotide, Structure, Genome, OMIM, Domains |
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PDB database(3D structure)
PROSITE Database of protein families and domains
Protein Mutant Database
Structural Classification of Proteins (SCOP)
Protein Structure Classification (CATH)
InterPro (database of protein families, domains and functional sites) |
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| Link to other database:
BRENDA
, EXPASY
, KEGG
, ERGO
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