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Enzyme Information: Zymolyase 20T (Lyticase, Yeast Lytic Enzyme)
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| Product name |
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Zymolyase 20T (Lyticase, Yeast Lytic Enzyme) |
| Cat# |
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Z1000 |
| Unit size |
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250mg |
| Prize |
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99 $  |
| Description |
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Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an essential enzyme responsible for lysis of viable yeast cell walls in this preparation is b-1,3-glucan-laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis .The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to the Zymolyase are obtained in the references.
Applications:
Protoplast/Spheroplast Preparation
Yeast Cell Fusion
Yeast Cell Transformation
Appearance: White, lyophilized powder
Activity: 20u/mg
Purity: Purified by ammonium sulfate precipitation.
Unit Definition:
One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800). Refer to Assay for Enzymatic Activity.
Synonyms: Lyticase*, Yeast Lytic Enzyme
Source: Arthrobacter luteus
Essential Enzyme: b-1,3-glucan laminaripentaohydrolase
Contaminants: Certified to be free of contaminating ribonuclease and deoxyribonuclease
Other activities contained:
b-1,3-glucanase: ~1.5x10e3u/mg
Protease: ~15u/mg
Mannanase: ~1.5x10e3u/mg
Amylase, xylanase, phosphatase: Minute amounts
DNase, Rnase: None detected
(See reference No. 3 as to the definition of each enzyme unit. Each activity varies more of less among lots.)
Optimum pH:
For cell wall lysis: 7.5, 35º
For yeast glucan hydrolysis: 6.5, 45º
Stable pH: 5-10
Heat Stability: The lytic activity is lost on incubation at 60º for 5 minutes.
Properties of Zymolyase:
Specificity (lytic spectrum)(5): Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomcopsis, Saccharomycodes, Schwanniomyces, etc
Susceptible strains in low concentration (0.2 units/ml):
Ashbya, Endomyces, Kloekera, Kluyveromyces, Pullularia, Saccharomyces
Susceptible strains in high concentration (2.0 units/ml):
Candida, Debaryomyces, Eremothecium, Hansenula, Hanseniaspora, Lipomyces, Metschnikowia, Saccharomycopsis, Saccharomycodes, Schizosaccharomyces, Selenozyma, Trigonopsis, Wickerhamia
Susceptibility depending upon strains:
Bretanomyces, Cryptococcus, Nadsonia, Picia, Rodosporidium, Schwanniomyces, Stephnoascus, Torulopsis
No susceptible strains:
Bullera, Pityosporum, Rhosotorula, Sporidiobolus, Sporobolomyces, Stetigmatomyces, Trichosporon
Activators: SH compound such as cystein, 2-mercaptoethanol of dithiothreitol
Storage and Stability:
Stable for 1 year at 4º. About 70% of the lytic activity is lost when stored at 30º for 3 months.
Companion Products:
Z1000: Zymolyase 20T
Z1001: Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol
Zymolyase 20T shows 20u/mg of the lytic activity, defined after, toward brewer's yeast cells (Saccharomyces cerevisiae, resting stage) or toward yeast cells of Saccharomyces cerevisiae IFO 0565 cultured statically in malt extract medium (Malt extract 2g, peptone 0.5g, water 100ml) at 20º for 34 hours.
Z1004: Zymolyase 100T
Z1005: Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol
Zymolyase 100T, further purified, whose specific activity is 100u/mg.
Note: Zymolyase100T is less soluble than 20T and may not be completely dissolved in buffers. If this is the case use as a suspension. |
| Request Info |
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| Supplier |
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United States Biological |
| Contact info |
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P.O. Box 261 Swampscott.
Massachusetts , United States
Phone:800 520 3011 |
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